Structural Dna Origami: Engineering Supermolecular Self-assembly for Nanodevice Fabrication
نویسنده
چکیده
............................................................................................................................ vi LIST OF TABLES .................................................................................................................... x LIST OF FIGURES ................................................................................................................. xi LIST OF ABBREVIATIONS .............................................................................................. xxiv CHAPTER 1: INTRODUCTION ............................................................................................. 1 CHAPTER 2: DNA ORIGAMI ................................................................................................ 9 2.1: DNA Structure ....................................................................................................... 9 2.2: DNA Origami Overview ...................................................................................... 15 2.2.1: Immobile Holliday Junction and Antiparallel Crossover ..................... 15 2.2.2: Two-Dimensional Scaffolded DNA Origami ....................................... 18 2.3: Six-Helix DNA Origami Nanotube ..................................................................... 23 2.3.1: Background ........................................................................................... 23 2.3.2: Design of the Bui et al. DNA Origami Nanotube................................. 25 2.3.3: Synthesis ............................................................................................... 32 CHAPTER 3: DIMERIZATION ............................................................................................ 38 3.1: Introduction .......................................................................................................... 38 3.2: Symmetrical DNA Origami Nanotube Head and Tail Heterodimer .................... 39 3.3: Yield for Head to Tail Dimers ............................................................................. 42
منابع مشابه
Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures
Self-assembly is a ubiquitous approach to the design and fabrication of novel supermolecular architectures. Here we report a strategy termed 'lipid-bilayer-assisted self-assembly' that is used to assemble DNA origami nanostructures into two-dimensional lattices. DNA origami structures are electrostatically adsorbed onto a mica-supported zwitterionic lipid bilayer in the presence of divalent cat...
متن کاملAutomated Quantification of the Impact of Defects on the Mechanical Behavior of Deoxyribonucleic acid Origami Nanoplates.
Deoxyribonucleic acid (DNA) origami is a method for the bottom-up self-assembly of complex nanostructures for applications, such as biosensing, drug delivery, nanopore technologies, and nanomechanical devices. Effective design of such nanostructures requires a good understanding of their mechanical behavior. While a number of studies have focused on the mechanical properties of DNA origami stru...
متن کاملOptimized assembly and covalent coupling of single-molecule DNA origami nanoarrays.
Artificial DNA nanostructures, such as DNA origami, have great potential as templates for the bottom-up fabrication of both biological and nonbiological nanodevices at a resolution unachievable by conventional top-down approaches. However, because origami are synthesized in solution, origami-templated devices cannot easily be studied or integrated into larger on-chip architectures. Electrostati...
متن کاملAn information-bearing seed for nucleating algorithmic self-assembly.
Self-assembly creates natural mineral, chemical, and biological structures of great complexity. Often, the same starting materials have the potential to form an infinite variety of distinct structures; information in a seed molecule can determine which form is grown as well as where and when. These phenomena can be exploited to program the growth of complex supramolecular structures, as demonst...
متن کاملDirect visualization of transient thermal response of a DNA origami.
The DNA origami approach enables the construction of complex objects from DNA strands. A fundamental understanding of the kinetics and thermodynamics of DNA origami assembly is extremely important for building large DNA structures with multifunctionality. Here both experimental and theoretical studies of DNA origami melting were carried out in order to reveal the reversible association/disassoc...
متن کامل